Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202,Thr185) Recombinant Rabbit Monoclonal Antibody (SZ2-4)
ERK1/2의 Thr202 및 Thr185 인산화 상태를 검출하는 고특이성 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 높은 로트 간 일관성과 민감도 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Thr185) Recombinant Rabbit Monoclonal Antibody (SZ2-4)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SZ2-4 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr185 of human Erk2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809318 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
ERK1 and ERK2 are widely expressed kinases involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. They are activated by various stimuli including growth factors, cytokines, viral infection, and G protein-coupled receptor ligands.
Upon activation:
- Ras interacts with Raf, activating it.
- Raf phosphorylates MEK1/2.
- MEK1/2 phosphorylates ERK1/2 on tyrosine/threonine residues.
- Activated ERK translocates to the nucleus and phosphorylates transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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