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Overview
Highly purified double-stranded cDNA
Clone genes directly by PCR, rather than library screening
Prepared from high-quality human tissues and cell lines
Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
More Information
Applications
Clone cDNAs without library screening
Generate hybridization probes using gene-specific or degenerate primers
Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
References
Lee, C. C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239, 1288–91 (1988).
Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246, 1620-2 (1989).
Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149–58 (1990).
Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57–61 (1990).
Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67–74 (1989).
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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