Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), PE, eBioscience
마우스 CD11b를 인식하는 M1/70 단클론 항체로 PE 형광 표지되어 있습니다. Mac-1 복합체 검출에 적합하며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 다양한 형광체 포맷 제공, 4°C 암소 보관, 연구용 전용 시약입니다.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 4 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 0.125 µg/test | 531 |
| In vitro Assay (IV) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Dog, Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465546 |
Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/BV series, eFluor dyes, FITC, Functional Grade, NovaFluor series, PE-Cyanine5/7, PE-eFluor 610, PerCP series, Super Bright series, and custom conjugation available.
Product Specific Information
Description:
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the Mac-1 (αMβ2 integrin) complex, binding to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity. M1/70 cross-reacts with human CD11b and detects this antigen on human monocytes, granulocytes, and some NK cells. CD11b participates in adhesive cell interactions.
Applications Reported:
Validated for flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using mouse splenocytes or bone marrow cells at ≤0.125 µg/test (per 100 µL final volume). Optimal titration recommended.
Excitation / Emission:
488–561 nm / 578 nm (Blue, Green, or Yellow-Green Laser compatible)
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18, known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 binds ICAM-1, ICAM-2, ICAM-4, CD14, CD23, fibrinogen, and Factor X, mediating cell adhesion and immune interactions. Expression levels vary by cell type and tissue; it serves as a microglial marker in nervous tissue. CD11b dysfunction is associated with systemic lupus erythematosus and related conditions.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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