Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), PE, eBioscience
마우스 CD11c 인식 단일클론 항체로 PE 형광표지되어 있으며, 주로 유세포분석(Flow Cytometry)에 사용됩니다. 덴드리틱 세포 및 활성화 T세포 표지에 적합하며, 0.5 µg/test 권장. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 4 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 5 |
| Immunocytochemistry (ICC/IF) | - | 3 |
| Flow Cytometry (Flow) | 0.5 µg/test | 266 |
| ELISA | - | 1 |
| Functional Assay | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465551 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–711
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- NovaFluor Blue 510–690
- NovaFluor Yellow 570
- PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- Custom conjugation available
Product Specific Information
Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion. N418 recognizes CD11c on splenic dendritic cells and precipitates a 150, 90 kDa heterodimer.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize concentration for specific assay.
Fluorescence Characteristics:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD11 forms a heterodimer adhesion molecule with CD18.
- CD11a: Expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, subset of lymphocytes
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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