Thermo Fisher Scientific Phospho-N-cadherin (Tyr820) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-860) and unphosphorylated N-cadherin (Tyr-820) peptides before affinity purification using phospho-N-cadherin (Tyr-820) peptide.
The purified antibody detects a 130 kDa band corresponding to N-cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but is not detected in untreated cells.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion.
Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species.
N-cadherin’s function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins.
The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.
Further, N-cadherin is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail.
The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.