Thermo Fisher Scientific N-cadherin (Tyr820) Polyclonal Antibody
N-cadherin (Tyr820) 특이적 폴리클로날 항체로, 인산화 상태 구분이 가능한 항체입니다. Western blot에 최적화되어 있으며 인간, 마우스, 랫트 시료에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Unphosphorylated N-Cadherin (Tyr-820) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in R-cadherin. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942887 |
Product Specific Information
This antibody was cross-adsorbed to phospho-N-cadherin (Tyr-820) peptide before affinity purification using unphosphorylated N-cadherin (Tyr-820) peptide.
The purified antibody detects a strong band at 130 kDa in western blots of serum-starved human endothelial cells, but the signal decreases in pervanadate-treated cells.
This reactivity is specifically blocked by the unphosphorylated N-cadherin (Tyr-820) peptide.
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion.
Cadherins regulate morphogenetic movements during development and control cell surface adhesion through homotypic interactions.
N-cadherin function depends on its association with the actin cytoskeleton via catenin proteins, with stability regulated by β-catenin phosphorylation and dephosphorylation.
It is a calcium-dependent cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
N-cadherin plays a crucial role during gastrulation and is required for the establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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