Thermo Fisher Scientific Blasticidin S Deaminase Polyclonal Antibody
Blasticidin S Deaminase 단백질을 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제됨. 안정적인 PBS/BSA/glycerol buffer에 보관되며, -20°C에서 장기 보존 가능.
- 카탈로그번호
- PA5143657
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Blasticidin S Deaminase Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fungi |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase* from Aspergillus terreus. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942885 |
Product Specific Information
This antibody detects a 13 kDa* protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector expressing the Bsd gene product. The band is not detected in mock-transfected cells.
Target Information
Retroviral vectors are commonly used for gene expression both in vitro and in vivo. These vectors typically contain a gene of interest and a selectable marker gene. Rapid selection without damaging cells is essential for efficient gene expression.
Blasticidin S deaminase (Bsd), isolated from Bacillus cereus, neutralizes the toxic antibiotic blasticidin. Many retroviral or lentiviral expression systems utilize Bsd expression for selection using blasticidin, enabling stable mammalian cell line selection within about one week.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.