Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Recombinant Rabbit Monoclonal Antibody (ERK12T202Y204-A11), FITC
FITC 형광 표지된 ERK1/2 인산화 특이 재조합 토끼 단일클론 항체로, Flow Cytometry에 최적화됨. 인간 및 생쥐 반응성. 높은 특이성과 재현성을 제공하며, Protein A/G 정제 및 PBS 버퍼에 보존됨. 연구용으로만 사용 가능.
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Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ERK12T202Y204-A11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745041 |
Additional Formats
- Unconjugated ([MA5-36895])
- APC ([MA5-36898])
- PE ([MA5-36896])
- Custom conjugation available
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to diverse rabbit immune repertoire
Target Information
ERK1 and ERK2 are widely expressed kinases involved in regulating meiosis, mitosis, and postmitotic functions in differentiated cells. These pathways are activated by various stimuli such as growth factors, cytokines, and viral infections.
Upon activation, ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors including c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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