Thermo Fisher Scientific Phospho-MEK1/MEK2 (Ser217, Ser221) Recombinant Rabbit Monoclonal Antibody (MEK12S217S221-H2)
인간 MEK1/MEK2의 Ser217/221 인산화 잔기를 인식하는 재조합 토끼 모노클로날 항체. Western blot 및 유세포 분석에 적합하며, 높은 특이성과 재현성을 제공. 단백질 A/G 정제, PBS-글리세롤 버퍼에 보존, 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-MEK1/MEK2 (Ser217, Ser221) Recombinant Rabbit Monoclonal Antibody (MEK12S217S221-H2)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | 1–1,000 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MEK12S217S221-H2 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser217/221 of human phospho MEK1/2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745042 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
MEK1 (MAP Kinase Kinase, also known as MKK) is a key component of the MAP kinase cascade regulating cell growth and differentiation. In the brain, this pathway also plays a critical role in synaptic signaling. Activated MEK1 acts as a dual specificity kinase phosphorylating both threonine and tyrosine residues on MAP kinase.
References:
Ahn, 1993; Chong et al., 2003; Adams and Sweatt, 2002; Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: MA5-28037_MEK12_Q02750-1_Rabbit.svg, MA5-28037_MEK12_P36507-1_Rabbit.svg 등)
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