Thermo Fisher Scientific E2F1 Recombinant Rabbit Monoclonal Antibody (JJ092-02)
E2F1 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC, Flow Cytometry에 적합합니다. 높은 특이성과 민감도를 제공하며 로트 간 일관성이 우수합니다. 단백질 A로 정제된 액상 형태로, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1000–1:5000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:1000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ092-02 |
| Immunogen | Synthetic peptide within Human E2F1 aa 371–404 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809753 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
The E2F1 protein is a member of the E2F family of transcription factors, which play a key role in cell cycle regulation and tumor suppressor protein activity.
E2F proteins contain conserved domains such as:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain (acidic amino acids)
- Tumor suppressor protein association domain
E2F1, along with E2F2 and E2F3, possesses an additional cyclin-binding domain and binds preferentially to the retinoblastoma protein (pRB) in a cell cycle-dependent manner. It mediates both cell proliferation and p53-dependent or independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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