Thermo Fisher Scientific 4EBP1 Recombinant Rabbit Monoclonal Antibody (JJ09-25)
Recombinant rabbit monoclonal antibody targeting 4EBP1 for WB, IHC, and ICC applications. High specificity and sensitivity with consistent lot performance. Suitable for human, mouse, and rat samples. Provided in liquid form, 1 mg/mL concentration, stor...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ09-25 |
| Immunogen | Synthetic peptide corresponding to Human eIF4EBP1 (aa 1–49) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809758 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
This gene encodes a translation repressor protein that interacts with eukaryotic translation initiation factor 4E (eIF4E), a key component in recruiting ribosomal subunits to mRNA. Interaction with eIF4E inhibits complex assembly and represses translation.
Phosphorylation of this protein in response to stimuli (e.g., UV irradiation, insulin signaling) leads to dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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