Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Super Bright 436, eBioscience
인간 CD8a 분자를 인식하는 OKT8 단클론 항체로, Super Bright 436 형광체 결합. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되고 436 nm에서 방출. T 세포 및 NK 세포 분석에 적합하며, 비특이적 결합 최소화를 위한 Super Bright Staining Buffer 사용 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/Test | View 10 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT8 (OKT-8) |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2762495 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin, eFluor 450, FITC
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow series (multiple wavelengths)
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
Description:
The OKT8 monoclonal antibody reacts with the human CD8a molecule (~32–34 kDa), expressed as CD8αβ heterodimer or CD8αα homodimer. CD8a is found on thymocytes, mature T cells, and NK cells. It binds MHC class I and associates with p56lck, playing a role in T-cell development and activation.
Preliminary testing shows OKT8, RPA-T8, and HIT8a clones do not compete for binding to human peripheral blood leukocytes, indicating distinct epitopes.
Applications Reported:
- Flow cytometric analysis of human peripheral blood cells.
Applications Tested:
- Pre-titrated for flow cytometry at 5 µL (0.25 µg) per test.
- Suitable for 10⁵–10⁸ cells/test in a 100 µL final volume.
Fluorochrome Information:
- Excitation: 405 nm
- Emission: 436 nm
- Recommended filter: 450/50 bandpass
- Ensure instrument capability for Super Bright 436 detection.
Buffer Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein involved in T cell signal transduction, differentiation, and activation.
CD8 is expressed on T cells as CD8αβ heterodimer or CD8αα homodimer and functions as a co-receptor with TCR for MHC class I molecules.
It is also found on subsets of NK cells, monocytes, and dendritic cells.
Activation via MHC-I/peptide complexes triggers Lck recruitment, cytokine production, and cytotoxic T lymphocyte (CTL) activity.
Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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