Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), Super Bright 436, eBioscience
인간 CD9 항원을 인식하는 eBioSN4 단클론 항체로 Super Bright 436 형광체가 결합되어 있습니다. 유세포분석에 최적화되어 있으며, 405 nm 레이저로 여기됩니다. 혈소판, B세포 등 다양한 세포에서 CD9 발현 연구에 적합합니다.
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Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioSN4 (SN4 C3-3A2)), Super Bright 436
Applications
- Western Blot (WB): Not tested
- Flow Cytometry (Flow): 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSN4 (SN4 C3-3A2) |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762499 |
Additional Formats
- Unconjugated
- APC
- Biotin
- FITC
- PE
- PerCP-eFluor 710
- Super Bright 645
- Custom conjugation available
Product Specific Information
The eBioSN4 monoclonal antibody reacts with human CD9, a 24 kDa tetraspanin family member characterized by four hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells, and neural cell lines. CD9 expression correlates with malignant phenotypes, including various leukemias.
In platelets, CD9 is associated with integrin αIIb/βIII and plays a role in platelet aggregation. It also mediates pre-B cell aggregation, adhesion, and migration of Schwann cells. CD9 provides co-stimulatory signals for T cells independently of CD28.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human platelets at 5 µL (0.25 µg) per test (100 µL final volume).
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD9 antigen is a glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells. It belongs to the tetraspanin family and regulates cell activation and aggregation via integrin CD41/CD61 interactions.
CD9 is involved in:
- Cell motility and adhesion
- Osteoclastogenesis and neurite outgrowth
- Myotube formation and sperm-egg fusion
- Suppression of cancer cell motility and metastasis
It plays critical roles in differentiation, signal transduction, and immune cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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