Thermo Fisher Scientific MDM2 Monoclonal Antibody (OTI16H3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
• For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. It is located on chromosome 12q.
Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, promoting p53 ubiquitination and degradation.
MDM2 phosphorylation also prevents its binding to p19ARF, further increasing p53 degradation.
Overexpression of MDM2 can inactivate p53, reducing its tumor suppressor function.
MDM2 participates in apoptosis and cell cycle regulation and is overexpressed in various human cancers, including soft tissue carcinomas and breast cancer.
It also interacts with other proteins such as retinoblastoma 1 and ribosomal protein L5.
More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.