Thermo Fisher Scientific TRPC5 Monoclonal Antibody (N67/15), APC
TRPC5 단백질을 특이적으로 인식하는 마우스 단클론 항체로, APC 형광표지 형태입니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 사용 가능합니다. Human, Mouse, Rat에서 반응하며, 단백질 검출 및 세포 분석 연구에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N67/15 |
| Immunogen | Synthetic peptide amino acids 827–845 of human TrpC5 (short transient receptor potential channel 5, Htrp5) |
| Conjugate | APC (Allophycocyanin) |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931931 |
Available Formats
Product Specific Information
1 µg/mL of MA5-45477 was sufficient for detection of TrpC5 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 110 kDa.
Target Information
TRPC5 belongs to the transient receptor potential (TRP) family and is one of seven mammalian TRPC proteins. It is a multi-pass membrane protein that forms receptor-activated, non-selective calcium-permeant cation channels.
TRPC5 can function alone or as a heteromultimer with TRPC1, TRPC3, and TRPC4. It interacts with proteins such as calmodulin, CABP1, enkurin, NHERF, MX1, RNF24, and SESTD1.
Activation is likely mediated by phosphatidylinositol second messenger systems linked to receptor tyrosine kinases or G-protein-coupled receptors. TRPC5 may also be activated by intracellular calcium store depletion.
Diseases associated with TRPC5 include Hypertrophic Pyloric Stenosis and Pyloric Stenosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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