Thermo Fisher Scientific TRPC5 Monoclonal Antibody (N67/15), FITC
TRPC5 단백질을 인식하는 FITC 결합 단클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 1 mg/mL 농도, 4°C 암소 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N67/15 |
| Immunogen | Synthetic peptide amino acids 827–845 of human TrpC5 (short transient receptor potential channel 5, Htrp5) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931932 |
Available Formats
- Unconjugated (MA5-27657)
- APC (MA5-45477)
- PE (MA5-45480)
- PerCP (MA5-45479)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45478 was sufficient for detection of TrpC5 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 110 kDa.
Target Information
TRPC5 belongs to the transient receptor potential (TRP) family and is one of seven mammalian TRPC proteins. It is a multi-pass membrane protein forming receptor-activated, non-selective calcium-permeant cation channels. TRPC5 can act alone or as a heteromultimer with TRPC1, TRPC3, and TRPC4. It interacts with calmodulin, CABP1, enkurin, NHERF, MX1, RNF24, and SESTD1. TRPC5 may be regulated by phosphatidylinositol second messenger systems activated by receptor tyrosine kinases or G-protein coupled receptors. It has been linked to calcium signaling and diseases such as hypertrophic pyloric stenosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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