Thermo Fisher Scientific TRPC5 Monoclonal Antibody (N67/15), PerCP
TRPC5 단백질을 검출하기 위한 PerCP 결합 단클론 항체. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 단백질 G로 정제된 액상 형태로 4°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N67/15 |
| Immunogen | Synthetic peptide amino acids 827–845 of human TrpC5 (short transient receptor potential channel 5, Htrp5) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931933 |
Available Formats
Product Specific Information
1 µg/mL of MA5-45479 was sufficient for detection of TrpC5 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 110 kDa.
Target Information
TRPC5 belongs to the transient receptor family and is one of seven mammalian TRPC (transient receptor potential channel) proteins. It is a multi-pass membrane protein that forms a receptor-activated, non-selective calcium-permeant cation channel. TRPC5 can act alone or as a heteromultimer with TRPC1, TRPC3, and TRPC4. It interacts with proteins such as calmodulin, CABP1, enkurin, NHERF, MX1, RNF24, and SESTD1. TRPC5 is likely regulated by phosphatidylinositol second messenger systems activated by receptor tyrosine kinases or G-protein coupled receptors and may also be activated by intracellular calcium store depletion. Diseases associated with TRPC5 include Hypertrophic Pyloric Stenosis and Pyloric Stenosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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