Thermo Fisher Scientific TRPC7 Monoclonal Antibody (N64A/36), PE
TRPC7 단백질을 인식하는 PE 결합 단클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 면역실험에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G로 정제된 액상 형태이며, 4°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N64A/36 |
| Immunogen | Synthetic peptide corresponding to amino acids 845–862 of human TRPC7 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931930 |
Available Formats:
- Unconjugated (MA5-27612)
- APC (MA5-45473)
- FITC (MA5-45474)
- PerCP (MA5-45475)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45476 was sufficient for detection of TRPC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 100 kDa.
Target Information
TRPC7 forms a receptor-activated, non-selective calcium-permeant cation channel. It is likely regulated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC7 is also activated by diacylglycerol (DAG) and may be influenced by intracellular calcium store depletion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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