Thermo Fisher Scientific TRPC7 Monoclonal Antibody (N64A/36), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N64A/36

Immunogen

Synthetic peptide amino acids 845-862 of human TRPC7 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRPC7,uniProtId:Q9HCX4-1,ncbiNodeId:9606,antigenRange:845-862,antigenLength:862,antigenImageFileName:MA5-45473_TRPC7_Q9HCX4-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45473_TRPC7_Q9HCX4-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

View additional formats

• Unconjugated
• FITC
• PE
• PerCP
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931927

Product Specific Information

1 µg/mL of MA5-45473 was sufficient for detection of TrpC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.| Detects approximately 100kDa.

Target Information

TRPC7 is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC7 is probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC7 is also activated by diacylglycerol (DAG). Further, TRPC7 may also be activated by intracellular calcium store depletion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.