Thermo Fisher Scientific TCR V beta 13 Monoclonal Antibody (MR12-3), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific TCR V beta 13 Monoclonal Antibody (MR12-3), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.125 µg/test

Product Specifications

Product Specific Information

This MR12-3 monoclonal antibody reacts with the T cell receptor (TCR) V beta 13 chain. TCR specificity is determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR12-3 antibody recognizes V beta 13 chains on T cells from mouse strains with the b haplotype of the Tcrb gene (e.g., C57BL/6, B10, C3H).
V beta 13⁺ T cells are deleted in mouse strains with the a (e.g., SJL) and c (e.g., RIIIS/J) haplotypes.

The MR12-3 monoclonal antibody has been reported to have blocking activity.

Applications Reported: Flow cytometric analysis and functional assays.
Applications Tested: Flow cytometric analysis of mouse splenocytes; ≤ 0.125 µg per test.
A test is defined as the amount of antibody that will stain a sample in 100 µL volume. Optimal titration is recommended.

Fluorophore Information:

• PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
• Excitation: 488 nm; Emission: 710 nm; Laser: Blue.
• Recommended filters: 685 LP dichroic mirror and 710/40 band pass (alternative: 695/40 band pass).
• Stable in 2% formaldehyde overnight at 4°C.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response.
Recognition of self-peptides is linked to autoimmune diseases; mutant peptides are associated with tumors.
Superantigen engagement can cause toxic shock syndrome.
Autoantibodies to V beta segments have been found in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating TH1-mediated inflammation.
Most human T cells express TCR αβ and either CD4 or CD8.
Double negative (DN) αβ T cells are increased in certain autoimmune and immunodeficiency disorders.
γδ T cells, mainly in epithelium, show less diversity and have antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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