Thermo Fisher Scientific TCR V beta 6 Monoclonal Antibody (RR4-7), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific TCR V beta 6 Monoclonal Antibody (RR4-7), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 4 publications

Product Specifications

Product Specific Information

This RR4-7 monoclonal antibody reacts with mouse V beta 6 TCR.
TCR specificity is determined by Va, Ja, Vb, Db, and Jb gene rearrangement.
The RR4-7 antibody recognizes the V beta 6 chain on T cells from mouse strains with the a (e.g., C57BR, SJL) and b haplotypes (e.g., BALB/c, C3H, DBA/1).
Strains expressing the Mls-1a antigen with MHC Class II I-E show fewer V beta 6 TCR+ T cells.

RR4-7 antibody demonstrates functional activity in vitro and in vivo.
Reported applications include flow cytometric analysis.
Tested by flow cytometric analysis of mouse splenocytes at ≤0.125 µg/test (100 µL volume).
Cell number may range from 10⁵ to 10⁸ cells/test.
Careful titration recommended for optimal performance.

PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Emission: 710 nm
• Excitation: 488 nm (Blue Laser)
  Recommended filter: 685 LP dichroic mirror and 710/40 band pass (or 695/40 BP alternative).

PerCP-eFluor® 710 conjugated antibodies are stable when samples are fixed with 2% formaldehyde overnight at 4°C.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, critical for adaptive immunity.
Self-peptide recognition is linked to autoimmune diseases, while mutant self-peptides are associated with tumors.
Superantigen engagement of TCRs can cause toxic shock syndrome.
Autoantibodies to V beta segments are found in RA and SLE patients, blocking TH1-mediated inflammation.
Most human T cells express TCR alpha-beta with CD4 or CD8 (single positive).
A small subset lacks both (double negative), increased in autoimmune and immunodeficiency disorders.
Gamma-delta T cells reside mainly in epithelia, showing less diversity and distinct antigen recognition, with antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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