Thermo Fisher Scientific TCR V alpha 3.2 Monoclonal Antibody (RR3-16), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific TCR V alpha 3.2 Monoclonal Antibody (RR3-16), PerCP-eFluor 710

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 3 publications

Product Specifications

Additional Formats

• APC
• FITC
• Request custom conjugation

Product Specific Information

This RR3-16 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 3.2 chain. TCR specificity is determined by Va, Ja, Vb, Db, and Jb gene rearrangement.
The RR3-16 antibody recognizes the V alpha 3.2 chain on T cells from mouse strains bearing the b (C57BL/6) or c haplotype (SJL, SWR, NOD) in the Tcra gene complex.
The V alpha 3.2 chain is absent in mice with a (Balb/c, AKR, C3H) and d (DBA/1, DBA/2) haplotypes.
Studies show that V alpha 3.2 TCR is more highly expressed on CD8+ T cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤ 0.125 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm
• Emission: 710 nm
• Laser: Blue Laser
  Recommended filter: 685 LP dichroic mirror and 710/40 band pass (695/40 acceptable alternative).

PerCP-eFluor® 710 conjugated antibodies are stable when samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules—critical for adaptive immune response.
TCR recognition of self-peptides relates to autoimmune disease and tumor formation.
Superantigen engagement can lead to toxic shock syndrome.
Autoantibodies to V beta segments are found in RA and SLE patients, potentially modulating inflammatory responses.
Most human T cells express TCR alpha-beta with CD4 or CD8; a minority are double negative (DN).
Gamma-delta T cells, mainly epithelial, show less diversity and distinct antigen recognition, with antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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