Thermo Fisher Scientific TCR V beta 12 Monoclonal Antibody (MR11-1), PerCP-eFluor 710, eBioscience
마우스 TCR V beta 12 체인을 인식하는 MR11-1 단클론 항체로, PerCP-eFluor 710 형광체 결합. Flow Cytometry용으로 최적화되어 있으며, C57BL/6 등 b haplotype 마우스에서 반응. 0.25 µg/test 권장, 4°C 암소 보관.
- 카탈로그번호
- 46-5798-80
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TCR V beta 12 Monoclonal Antibody (MR11-1), PerCP-eFluor 710, eBioscience
Thermo Fisher Scientific TCR V beta 12 Monoclonal Antibody (MR11-1), PerCP-eFluor 710, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MR11-1 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10852552 |
Product Specific Information
This MR11-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 12 chain. The MR11-1 antibody recognizes the V beta 12 chain on T cells from mouse strains with the b haplotype (e.g., C57BL/6, B10, C58/J). V beta 12+ T cells are absent in strains with the a (SJL) or c (RIII) haplotypes. Strains expressing Mls antigens (BALB/c, C3H) show fewer V beta 12+ T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse lymph node cells at ≤0.25 µg/test (100 µL final volume). Cell number: 10^5–10^8 cells/test. Optimize titration for best performance.
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Emission: 710 nm
- Excitation: 488 nm (Blue Laser)
- Recommended filters: 685 LP dichroic mirror + 710/40 band pass filter (alternative: 695/40 BP)
PerCP-eFluor® 710 conjugates are stable when samples are fixed with 2% formaldehyde overnight at 4°C.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, crucial for adaptive immune response. Recognition of self-peptides is linked to autoimmune diseases; mutant peptides are associated with tumors. Superantigen engagement of TCRs causes toxic shock syndrome. Autoantibodies to V beta segments appear in rheumatoid arthritis and lupus, modulating TH1-mediated inflammation.
Most human T cells express TCR alpha-beta and CD4 or CD8 (SP). Some are double negative (DN), increased in certain autoimmune disorders. Gamma-delta T cells, mainly epithelial, show limited diversity and unique antigen recognition, with antitumor and immunoregulatory roles.
For Research Use Only. Not for diagnostic use or resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.