Thermo Fisher Scientific beta-NGF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human beta-NGF. Anti-Human beta-NGF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human beta-NGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human beta-NGF (500-P85) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human beta-NGF.

Western Blot:
To detect Human beta-NGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Human beta-NGF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P85BT-1MG will be provided as 2 × 500 µg.

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of various neuronal and non-neuronal cells. NGF binds to specific cell surface receptors that exist as high and low affinity sites. Only the high affinity receptor mediates signal transduction.
Cross-linking studies with 125I-NGF show that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF, while the low affinity receptor forms an Mr 100,000 complex.
Human and rat NGF receptor genes share over 90% homology. NGF promotes nerve growth and differentiation of sympathetic and sensory neurons.
Mutations in the NGF gene are linked to hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulated NGF expression is associated with allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.