Thermo Fisher Scientific Leptin Polyclonal Antibody, Biotin, PeproTech
휴먼 렙틴(Leptin)에 특이적인 Biotin 접합 폴리클로날 항체. Western blot과 Sandwich ELISA에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제됨. 동결건조 형태로 제공되며, -20°C에서 보관. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P86BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Leptin Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Leptin |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930819 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Leptin. Anti-Human Leptin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human Leptin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Leptin (500-P86) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Leptin.
Western Blot:
To detect hLeptin by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hLeptin is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P86BT-1MG will be provided as 2 × 500 µg.
Target Information
Leptin is a 167 amino acid protein hormone (~16 kDa) encoded by the ob gene. It regulates body weight, metabolism, and reproductive function. Expressed mainly by adipocytes, leptin levels correlate with body fat mass. It also plays roles in hematopoiesis, angiogenesis, wound healing, inflammation, immune response, and energy homeostasis. Defects in leptin production can cause severe hereditary obesity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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