Thermo Fisher Scientific IDH1 Polyclonal Antibody
IDH1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. Human, Mouse, Rat에 반응하며, 고순도 정제 및 안정적인 액상 형태로 제공. 연구용으로 세포 내 NADPH 생성 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 116–143 from the central region of human IDH1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion - Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2280117 |
Product Specific Information
This antibody is predicted to react with bovine and rat based on sequence homology.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes are divided into NAD(+)-dependent and NADP(+)-dependent subclasses. Five isocitrate dehydrogenases have been identified: three NAD(+)-dependent forms in the mitochondrial matrix, and two NADP(+)-dependent forms—one mitochondrial and one cytosolic. The NADP(+)-dependent cytosolic isozyme encoded by this gene is also present in peroxisomes, where it contributes to NADPH regeneration for intraperoxisomal reductions and other metabolic processes such as alpha-hydroxylation of phytanic acid. This enzyme is essential for maintaining cytoplasmic NADPH levels.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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