Thermo Fisher Scientific PI3K p85/p55 Recombinant Superclonal Antibody (6HCLC)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-4 µg

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

6HCLC

Immunogen

Peptide corresponding to amino acids 369-382 of human PI3K. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI3K p85/p55,uniProtId:P27986-1,ncbiNodeId:9606,antigenRange:369-382,antigenLength:724,antigenImageFileName:710400_PI3K_p85p55_P27986-1_Rabbit.svg,antigenImageFileNamePDP:710400_PI3K_p85p55_P27986-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532708

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.