Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), eFluor 450, eBioscience
TNKUPJ 클론의 Rat IgG2a 항체로 마우스 CD163 단백질을 인식합니다. eFluor 450 형광체 결합으로 유세포 분석에 적합하며, 고정·투과된 세포 내 CD163 검출 가능. 405 nm 레이저로 여기, 445 nm에서 방출. 연구용으로만 사용.
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Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 13 publications |
Product Specifications
| Item | Description |
|---|---|
| Published species | Not Applicable |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TNKUPJ |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2815179 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- APC
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by specific subsets of tissue macrophages such as splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages, and a small fraction of peritoneal macrophages. Mouse monocytes do not express CD163, and it is not a reliable marker for M2 macrophages.
This antibody detects CD163 on fixed and permeabilized cells, enabling intracellular staining. Aggressive tissue dissociation may reduce surface CD163; intracellular detection is recommended in such cases.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.125 µg per test (100 µL final volume). Optimal titration is recommended.
Fluorophore Information:
eFluor 450 is an alternative to Pacific Blue, excited at 405 nm and emits at 446 nm. Ensure instrument compatibility with violet laser excitation.
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and a receptor for the hemoglobin-haptoglobin complex. It protects tissues from oxidative damage and aids iron recycling through endocytosis. CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and shed upon LPS or PMA stimulation. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, triggering intracellular signaling cascades.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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