Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), eFluor 450, eBioscience
마우스 CCR7(CD197)을 인식하는 4B12 클론 단일클론 항체로, eFluor 450 형광표지되어 있습니다. 흐름세포분석(Flow Cytometry)에 최적화되어 있으며, 37°C에서 더 높은 형광 강도를 제공합니다. 면역세포 이동 및 림프구 분화 연구에 적합합니다.
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Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 21 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B12 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1944352 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), a chemokine receptor for CCL19 and CCL21. CCR7 mediates lymphocyte migration into secondary lymphoid organs and plays a role in dendritic and B cell localization during immune responses.
Human CCR7 also marks central memory T cells (TCM), which express CCR7 and CD62L and reside in peripheral lymphoid organs. CCR7 contributes to thymocyte development, and its deficiency leads to disrupted thymic architecture and T cell development.
Staining Protocol
- Prepare cell suspension and block Fc receptors using purified anti-mouse CD16/32 (5 µg/million cells) for 15 min on ice.
- Without washing, add 4B12 antibody (1 µg/million cells) and incubate at 37°C or 2–8°C for 30 min.
- Wash cells once with 3 mL Flow Cytometry Staining Buffer and decant supernatant.
- Analyze by flow cytometry or proceed with secondary staining.
Note: Staining at 37°C yields brighter fluorescence and better resolution. Co-staining with CCL19-Fc may be hindered due to steric interference.
Fluorophore Information
- eFluor® 450 is an alternative to Pacific Blue®
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CCR7 is a G protein-coupled chemokine receptor induced by Epstein-Barr virus (EBV). It is expressed in blood, bone marrow, lymph node, and intestine, particularly in activated B and T cells. CCR7 regulates migration of memory T cells and dendritic cell maturation. Its specific ligand is CCL19/ECL.
For Research Use Only. Not for use in diagnostic procedures.
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