Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eFluor 450, eBioscience

Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eFluor 450, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen-presenting cells including B cells, monocytes/macrophages, and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with cynomolgus monkey CD154.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of stimulated peripheral blood cells. Use at 5 µL (0.25 µg) per test. A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®. It emits at 445 nm and is excited with the Violet laser (405 nm). Ensure your instrument can detect this fluorochrome.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. It stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand induces cytokine production and tumoricidal activity in peripheral blood monocytes and co-stimulates proliferation of activated T cells, accompanied by IFN-γ, TNF-α, and IL-2 production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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