Thermo Fisher Scientific SSB Monoclonal Antibody (OTI3F11), TrueMAB
SSB 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, IP 등에 사용 가능. Human 및 Dog 시료에 반응하며, Lyophilized 형태로 제공. RNA 대사 관련 연구 및 Sjogren 증후군 관련 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F11 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human SSB expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
La protein (SSB) plays a role in multiple RNA metabolic processes, including:
- Binding and protecting 3′ UUU(OH) elements of RNA polymerase III transcripts
- Processing 5′ and 3′ ends of pre-tRNA precursors
- Acting as an RNA chaperone
- Binding viral RNAs, including those associated with hepatitis C virus
La protein was originally characterized by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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