Thermo Fisher Scientific BRAF Monoclonal Antibody (OTI1G6), TrueMAB
BRAF 단백질을 인식하는 Mouse monoclonal antibody로 Western blot과 Immunoprecipitation에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. Affinity chromatography로 정제되어 고순도 확보. 연구용으로 MAPK/ERK 신호전달 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G6 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human BRAF expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
BRAF is involved in transducing mitogenic signals from the cell membrane to the nucleus and is activated by somatic point mutations in human cancers. It regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.
Mutations in BRAF are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma.
A pseudogene for BRAF is located on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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