Thermo Fisher Scientific BRAF Monoclonal Antibody (OTI4B2), TrueMAB
인간 BRAF 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot, ELISA, IP에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. MAPK/ERK 경로 연구 및 암 관련 BRAF 변이 분석에 적합.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000 | |
| ELISA (ELISA) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B2 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human BRAF expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutations in human cancer and plays a role in postsynaptic responses of hippocampal neurons.
This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.
Mutations in B-Raf are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma.
A pseudogene for B-Raf is located on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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