Thermo Fisher Scientific CD11b Monoclonal Antibody (C67F154), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Request custom conjugation

Product Specific Information

The C67F154 monoclonal antibody reacts with human CD11b. CD11b (integrin alpha-M) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex, also known as integrin αMβ2, Mac-1, or CR3, is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK and dendritic cells, and microglia.

CD11b/CD18 functions as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X — interactions critical for cell-cell and cell-matrix adhesion.

This antibody has been tested in IHC of formalin-fixed paraffin-embedded tissues using low or high pH antigen retrieval at ≤5 µg/mL. Optimal dilution should be determined by the user.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 to create Mac-1/CR3, a receptor involved in immune cell adhesion and migration. It is expressed on monocytes, macrophages, granulocytes, NK cell subsets, dendritic cells, and microglia. Expression levels vary by cell type and maturation state, with high levels in peritoneal macrophages and lower levels in splenic macrophages.

CD11b is a key marker for microglia in neural tissue and plays roles in immune responses and inflammation. Dysregulation is associated with conditions such as systemic lupus erythematosus and ITGAM-related autoimmune susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.