Thermo Fisher Scientific Furin Polyclonal Antibody

Thermo Fisher Scientific Furin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1-062 detects furin convertase from canine and mouse cells as well as transfected human furin.
This antibody does not detect endogenous furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells, or from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.

PA1-062 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation, and immunofluorescence procedures.
By Western blot, this antibody detects an ~100 kDa protein representing furin in BSC-40 cells transfected with the human furin gene.
In addition, PA1-062 detects human furin transfected into 3T3 cells.
Immunofluorescence staining of endogenous furin convertase in 3T3 cells with PA1-062 yields a pattern consistent with paranuclear staining.

The PA1-062 immunogen is a synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin.
The PA1-062 immunizing peptide (Cat. # PEP-010) is available for use in neutralization and control experiments.

Target Information

Furin is a membrane-associated, calcium-dependent serine protease belonging to the subtilisin-like prohormone convertase (PC) family.
Members of this family cleave most prohormones and neuropeptide precursors, as well as various cellular, viral, and bacterial proteins transported via the constitutive secretory pathway.

Furin and other PC family members share structural similarities, including:

• ~10 kDa amino-terminal proregion
• ~55 kDa subtilisin-like catalytic domain
• Variable carboxyl-terminal domain

These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment.
Furin is the only known PC to possess a transmembrane domain.
Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence RX(K/R)R.
The acidic peptide sequence C771PSDSEEDEG780 localizes furin to the trans-Golgi-network, and phosphorylation of serine residues within this region modulates intracellular routing.
An additional signaling domain, Y759KGL762, directs internalization from the cell surface.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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