Thermo Fisher Scientific Furin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 23 publications 23 publications

Immunoprecipitation (IP)

1:100

View 3 publications 3 publications

Neutralization (Neu)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Furin,uniProtId:P09958-1,ncbiNodeId:9606,antigenRange:780-793,antigenLength:794,antigenImageFileName:PA1-062_Furin_P09958-1_Rabbit.svg,antigenImageFileNamePDP:PA1-062_Furin_P09958-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2105077

Product Specific Information

PA1-062 detects furin convertase from canine and mouse cells as well as transfected human furin. This antibody does not detect endogenous furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells, or from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.

PA1-062 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects an ~100 kDa protein representing furin in BSC-40 cells transfected with the human furin gene. In addition, PA1-062 detects human furin transfected into 3T3 cells. Immunofluorescence staining of endogenous furin convertase in 3T3 cells with PA1-062 yields a pattern consistent with paranuclear staining.

The PA1-062 immunogen is a synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin. The PA1-062 immunizing peptide (Cat. # PEP-010) is available for use in neutralization and control experiments.

Target Information

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.