Thermo Fisher Scientific alpha COP Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

PA1-067 detects coatomer-protein I alpha (COPI alpha) from human and rat cells.
It has been successfully used in Western blot and immunocytochemistry procedures.
By Western blot, this antibody detects an ~140 kDa protein representing COP alpha from PC-12 cell extract, and also an unidentified protein at ~100 kDa.
Immunocytochemical staining of alpha-COP using PA1-067 shows localization to the Golgi complex and distinct vesicular structures throughout the cytoplasm.

PA1-067 immunogen is a synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP.
The PA1-067 immunizing peptide (Cat. # PEP-125) is available for use in neutralization and control experiments.

Target Information

In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs).
Seven coat proteins have been identified, representing subunits of a complex known as coatomer: alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP.
The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast.
The N-terminal 25 amino acids of alpha-COP encode the bioactive peptide xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone.
Alternative splicing results in multiple splice forms encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.