Thermo Fisher Scientific beta COP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 42 publications 42 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Hamster, Human, Mouse, Non-human primate, Rat, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta COP,uniProtId:P23514-1,ncbiNodeId:10114,antigenRange:497-513,antigenLength:953,antigenImageFileName:PA1-061_beta_COP_P23514-1_Rabbit.svg,antigenImageFileNamePDP:PA1-061_beta_COP_P23514-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2081296

Product Specific Information

PA1-061 detects Golgi beta-coatomer protein (beta-COP) from mouse, human and rat tissues.

PA1-061 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110kDa protein which represents beta-COP from rat brain homogenate. Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells with PA1-061 results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

The PA1-061 immunogen is a synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP. This sequence is completely conserved in human beta-COP.

Target Information

This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.