Thermo Fisher Scientific beta COP Polyclonal Antibody
Thermo Fisher Scientific의 beta COP Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 Golgi beta-coatomer 단백질을 검출하는 항체입니다. WB, IHC, ICC/IF 등 다양한 실험에 적용 가능하며, 고순도 정제 및 안정적 저장 조건을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 11 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 42 publications |
| Immunoprecipitation (IP) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Bovine, Hamster, Human, Mouse, Non-human primate, Rat, Yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2081296 |
Product Specific Information
PA1-061 detects Golgi beta-coatomer protein (beta-COP) from mouse, human, and rat tissues.
It has been successfully used in Western blot and immunofluorescence procedures.
By Western blot, this antibody detects an ~110 kDa protein representing beta-COP from rat brain homogenate.
Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells shows localization to the Golgi complex and vesicular structures throughout the cytoplasm.
Golgi localization of beta-COP is sensitive to brefeldin A treatment or ATP depletion.
The immunogen is a synthetic peptide corresponding to residues E(496)-K(513) of rat beta-COP, which is completely conserved in human beta-COP.
Target Information
This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles.
The coatomer complex (coat protein complex 1, COPI) forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases.
At the Golgi membrane, it assists in the movement of protein and lipid components back to the endoplasmic reticulum.
Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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