Thermo Fisher Scientific c-Myc Monoclonal Antibody (OTI3F2), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is frequently activated in tumor cells and plays a critical role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation at Thr58/Ser62 by kinases such as glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK regulates its function in proliferation and cell cycle control.
c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene (p62 c-myc) controls normal cellular proliferation and differentiation, while deregulated expression induces apoptosis. Antibodies against c-myc epitopes recognize overexpressed Myc-tagged proteins at either the N- or C-terminus.

c-Myc, N-Myc, and L-Myc proteins are involved in cell proliferation, differentiation, and tumorigenesis. Amplification of c-Myc is found in several human cancers, including lung, breast, and colon carcinomas. N-Myc amplification is observed in neuroblastomas.
Translocation of the c-myc locus on chromosome 8 to immunoglobulin loci (chromosomes 14, 2, or 22) is characteristic of Burkitt’s lymphoma and other B-cell lymphoproliferative disorders. Aberrant c-myc expression occurs in various tumors such as colorectal, gastric, hepatic, mammary, ovarian, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.