Thermo Fisher Scientific AKT3 Monoclonal Antibody (OTI9B2), TrueMAB
AKT3 단백질을 인식하는 Mouse 모노클로날 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. Human 및 Mouse 반응성. Lyophilized 형태로 제공되며, 고순도 Affinity chromatography 정제. 연구용으로 세포 신호전달 및 종양 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9B2 |
| Immunogen | Full length human recombinant protein of human AKT3 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
AKT3 (PKB gamma) is activated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
It plays roles in various biological processes including:
- Cell proliferation and differentiation
- Apoptosis and tumorigenesis
- Glycogen synthesis and glucose uptake
Alternative splice variants encoding distinct isoforms have been described.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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