Thermo Fisher Scientific p53 Recombinant Rabbit Monoclonal Antibody (PD01-32)
Recombinant rabbit monoclonal antibody for human p53 detection. Validated for IHC (paraffin) with high specificity. Recognizes full-length human p53 protein. Suitable for cancer and cell cycle research. For research use only.
- 판매단위
- pk
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Applications
Tested Applications and Dilutions
- Immunohistochemistry (Paraffin) (IHC (P)): 1:1,000–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PD01-32 |
| Immunogen | Recombinant full-length protein within Human p53 (aa 1–393/393) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3092965 |
Product Specific Information
- Positive Control: Human stomach cancer tissue, human breast cancer tissue
- Predicted Band Size: 53 kDa
- Subcellular Location: Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. p53 upregulates genes such as p21waf1, leading to G1 or G2/M arrest.
The protein structure includes:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerisation domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is critical for activation and stabilization. p53 helps prevent proliferation of damaged cells and acts as an anticancer agent. Phosphorylation at Ser392 correlates with tumor formation. Increased p53 levels are linked to aging, oxidative stress, and cognitive deficits related to long-term potentiation.
Mutations in TP53 lead to loss of tumor suppressor function and are common in various cancers. Germline mutations can occur in cancer-prone families, such as those with Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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