Thermo Fisher Scientific p53 Recombinant Rabbit Monoclonal Antibody (PD01-32)

Applications

Tested Applications and Dilutions

• Immunohistochemistry (Paraffin) (IHC (P)): 1:1,000–1:2,000

Product Specifications

Product Specific Information

• Positive Control: Human stomach cancer tissue, human breast cancer tissue
• Predicted Band Size: 53 kDa
• Subcellular Location: Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. p53 upregulates genes such as p21waf1, leading to G1 or G2/M arrest.
The protein structure includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization. p53 helps prevent proliferation of damaged cells and acts as an anticancer agent. Phosphorylation at Ser392 correlates with tumor formation. Increased p53 levels are linked to aging, oxidative stress, and cognitive deficits related to long-term potentiation.
Mutations in TP53 lead to loss of tumor suppressor function and are common in various cancers. Germline mutations can occur in cancer-prone families, such as those with Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.