Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (ISA-3), eFluor 450, eBioscience
인간 ICOS(CD278)를 인식하는 ISA-3 단클론 항체로, eFluor 450 형광체로 결합되어 유세포분석에 최적화됨. 활성화된 T세포의 공동자극 분자 검출에 사용. 405 nm 레이저에서 여기, 445 nm에서 방출. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 9 publications |
| Neutralization (Neu) | - | View 1 publication |
| Functional Assay (Functional) | - | View 1 publication |
| T-Cell Activation (TCA) | - | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ISA-3 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_10853193 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- FITC
- Functional Grade
- NovaFluor Red 660
- NovaFluor Red 710
- NovaFluor Yellow 700
- NovaFluor Yellow 730
- PE
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1, and AILIM.
ICOS is a T cell-specific activation molecule and a member of the CD28/CTLA-4 family. It plays a crucial role in T cell activation and humoral immune responses, particularly in memory B cell and plasma cell generation.
- ICOS binds to its ligand B7h/B7RP-1 on activated APCs and inflamed tissues.
- Plate-bound ISA-3 is costimulatory for T cells and induces cytokine production (IL-4, IL-5, IL-10), but not IL-2.
- Generated against human ICOS antigen.
- C398.4A (anti-mouse ICOS/H4) cross-reacts with human ICOS but binds a different epitope.
- For brightest staining of activated human T cells, use Product #13-9948-82 or #12-9948-42.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells.
Use at 5 µL (0.25 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm (Violet laser). Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ICOS (CD278) encodes a cell-surface receptor of the CD28/CTLA-4 family.
It functions as a homodimer and is essential for immune cell signaling, proliferation, and regulation.
Mutations or dysregulation are linked to immunodeficiency disorders such as Common Variable Immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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