Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), eFluor 450, eBioscience
인간 TLR4 인식용 HTA125 단클론 항체로, eFluor 450 형광 표지되어 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 405 nm 레이저로 여기되며 445 nm에서 방출합니다. 선천면역 연구 및 TLR4 발현 분석에 적합합니다.
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), eFluor 450, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | 16 |
| ELISA | - | 1 |
| Neutralization | - | 7 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HTA125 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848395 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). TLR4 is a type I transmembrane protein involved in innate immune responses. It forms a complex with MD-2 and CD14 to recognize and signal in response to bacterial lipopolysaccharides (LPS). TLR4 is primarily expressed on peripheral blood monocytes and at lower levels on granulocytes and immature dendritic cells.
- Recommended staining method: Lysed whole blood protocol for optimal TLR4 detection.
- Density gradient separation can reduce staining intensity.
- Applications Reported: Flow cytometric analysis.
- Tested Application: Flow cytometry using 5 µL (0.5 µg) per test (in 100 µL final volume).
- Suitable for detection of 10⁵–10⁸ cells/test.
- eFluor 450 emits at 446 nm and is excited by the 405 nm violet laser line.
- Ensure instrument compatibility with eFluor 450 fluorochrome.
Excitation: 405 nm
Emission: 445 nm
Laser: Violet Laser
Target Information
TLR4 (Toll-like receptor 4) is part of the TLR family responsible for pathogen recognition and activation of innate immunity. It is highly expressed in placenta and myelomonocytic leukocytes. TLR4 mediates responses to lipopolysaccharide (LPS) from Gram-negative bacteria by forming a receptor complex with CD14 and LY96 (MD-2). Mutations in TLR4 can alter LPS responsiveness. It is expressed in peripheral blood monocytes, a subset of B cells, and placenta.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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