Thermo Fisher Scientific HLA-DR Monoclonal Antibody (L243), eFluor 450, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 20 publications

Product Specifications

Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The L243 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class II molecule, HLA-DR.
HLA-DR is expressed on antigen-presenting cells (APCs) including B cells, monocytes, macrophages, dendritic cells, and activated T cells. It is a heterodimeric transmembrane protein composed of alpha and beta subunits that play an essential role in presenting peptides to CD4⁺ T lymphocytes.

The L243 antibody recognizes a different epitope than LN3 and does not cross-block binding.

• Reported Application: Flow cytometric analysis
• Tested Application: Flow cytometry using normal human peripheral blood cells
• Recommended Use: 5 µL (0.125 µg) per 100 µL sample (10⁵–10⁸ cells/test)

Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure the cytometer supports Violet laser detection.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).

• The alpha chain gene (DRA) contains 5 exons:
  • Exon 1: leader peptide
  • Exons 2–3: extracellular domains
  • Exon 4: transmembrane domain and cytoplasmic tail
• DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
• The beta chain (DRB) carries polymorphisms that define peptide-binding specificity.
• Hundreds of DRB1 alleles exist and are routinely typed in bone marrow and kidney transplantation.

HLA-DR is primarily expressed on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
MHC class II molecules (DR, DQ, DP) bind intracellularly processed peptides, present them to T-helper cells, and play a critical role in immune response initiation.

Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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