Thermo Fisher Scientific TGF beta-1,2,3 Monoclonal Antibody (eBio16TFB), Biotin, eBioscience
TGF beta-1,2,3 단백질을 인식하는 Biotin 결합 단일클론 항체로, ELISA 분석에 적합합니다. 인간 및 마우스 시료에 반응하며, 높은 특이성과 재현성을 제공합니다. 연구용으로 TGF-beta 관련 면역학적 분석에 활용 가능합니다.
- 카탈로그번호
- 13-9923-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TGF beta-1,2,3 Monoclonal Antibody (eBio16TFB), Biotin, eBioscience
Thermo Fisher Scientific TGF beta-1,2,3 Monoclonal Antibody (eBio16TFB), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | - | View 1 publication |
| ELISA | 2–6 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio16TFB |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1271971 |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The eBio16TFB antibody reacts with Transforming Growth Factor-beta (TGF-beta), a pleiotropic cytokine existing in five isoforms (TGF-β1–5) with 70–80% homology among them. TGF-β1 is the most abundant form, widely expressed in lymphoid organs, while other isoforms have more restricted expression. All isoforms are biologically active as disulfide-linked homodimers.
TGF-β1 is produced at high levels by platelets and also by macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. Its secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. TGF-β1 inhibits proliferation of various cell types including T cells and NK cells, and downregulates macrophage activity. It also blocks IL-2–mediated anti-tumor activity of lymphokine-activated killer (LAK) cells.
Recent studies show TGF-β1 plays a key role in regulatory T cell development. Dendritic cells exposed to tumors secrete TGF-β1, promoting expansion of Treg cells and inducing Foxp3 expression, leading to differentiation of CD4⁺CD25⁺ Treg cells (Ti-Treg).
Applications Reported: Suitable for use in ELISA.
Applications Tested: Used as detection antibody in sandwich ELISA for human and mouse TGF-beta, in combination with capture antibody eBioTB2F (Product #14-9943-81). Recommended concentration range: 2–6 µg/mL. Include a standard curve from 8000 pg/mL to 60 pg/mL per plate.
This antibody recognizes the mature/active form of TGF-beta1 (not associated with LAP). Most samples require acid treatment and neutralization to remove LAP prior to analysis. Some serum/plasma samples may contain low levels of free TGF-beta1 measurable without acid treatment.
Target Information
TGF-beta1, -2, and -3 are closely related proteins (70–80% sequence homology) produced by many cell types, functioning as growth and differentiation factors. The active forms are disulfide-linked homodimers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.