Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (C398.4A), Biotin, eBioscience

Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (C398.4A), Biotin, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 7 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• Super Bright 436
• Request custom conjugation

Product Specific Information

The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell-specific activation molecule, a member of the CD28/CTLA-4 family, expressed on activated T cells. It plays a key role in T cell activation, proliferation, and humoral immune response.

ICOS binds to its ligand B7h/B7RP-1 on activated APCs, including B cells, and is involved in Th2 cell development and autoimmune regulation.
C398.4A displays strong costimulation of T cell clone proliferation, intermediate activation on activated T cells, and weak activation on resting T cells. It enhances IL-10 production from pre-activated cells expressing high levels of ICOS. The epitope recognized by C398.4A is distinct from that bound by ICOSL (does not block ICOSL binding).

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on Con A-stimulated mouse splenocytes at ≤0.125 µg/test (100 µL volume). Optimal titration recommended.

Target Information

ICOS (CD278) encodes a protein in the CD28 and CTLA-4 receptor family. It functions as a homodimer and is crucial for cell-cell signaling, immune responses, and regulation of proliferation. ICOS has been associated with disorders such as Common Variable Immunodeficiency (CVID).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.