Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Biotin, eBioscience
마우스 TLR4/MD-2 복합체를 인식하는 Biotin-conjugated 단클론 항체로, 선천면역 연구에 적합. Flow cytometry 및 IHC(Frozen) 등 다양한 응용 가능. 0.5 µg/test 권장, 4°C 보관. Affinity chromatography로 정제된 고품질 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 0.5 µg/test | 11 |
| Neutralization (Neu) | - | 2 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MTS510 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466991 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
Product Specific Information
The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. The Toll family of type I transmembrane proteins includes receptors with extracellular leucine-rich repeats and cytoplasmic domains homologous to the type I IL-1 receptor. TLR2 and TLR4 are key pattern recognition receptors involved in innate immunity and inflammation.
TLR4 forms a complex with MD-2 and CD14 to mediate LPS recognition and signaling. In mice, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages, and its surface expression decreases upon LPS stimulation. Defective TLR4 genes in C3H/HeJ and C57BL/10ScCr mice result in hyporesponsiveness to LPS.
The MTS510 antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), preferentially binding to the TLR4/MD-2 complex. In contrast, UT41 antibody binds regardless of complex formation.
Applications Reported: Flow cytometry and immunohistochemical staining of frozen tissue sections.
Applications Tested: Tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. Recommended ≤0.5 µg/test (100 µL final volume). Cell density may range from 10⁵–10⁸ cells/test. Optimize concentration for specific assay conditions.
Target Information
MD-2 is part of the Toll/interleukin-1 receptor (TIR) family, which includes Toll-like receptors (TLRs) that recognize pathogen-associated molecular patterns (PAMPs). TLR4, the main receptor for lipopolysaccharide (LPS), requires MD-2 binding for maximal signaling. The LPS response specificity depends on MD-2 species; human MD-2 expression in mouse cells can alter TLR4 ligand specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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