Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (ISA-3), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 660
• NovaFluor Red 710
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1, and AILIM. ICOS is a T cell-specific activation molecule and a member of the CD28/CTLA-4 family. It has a molecular mass of 55–60 kDa, composed of 27 kDa and 29 kDa chains.

ICOS plays a critical role in T cell activation and humoral immune responses, particularly in memory B cell and plasma cell generation. It binds to its ligand B7h/B7RP-1 on activated antigen-presenting cells and inflamed tissues.

Plate-bound ISA-3 provides costimulatory signals for T cells, inducing cytokines such as IL-4, IL-5, and IL-10, but not IL-2. ISA-3 has the same reactivity as F44 and was generated against human ICOS antigen.

C398.4A, anti-mouse ICOS/H4 (Product # 14-9949-82), cross-reacts with human ICOS but binds a different epitope. For brightest staining of ICOS on activated human T cells, use Product # 13-9948-82 or Product # 12-9948-42.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on unstimulated and CD3/CD28-stimulated human blood cells (3 days). Use ≤0.5 µg/test in 100 µL final volume. Optimize antibody titration for best performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that functions as a homodimer and is part of the CD28/CTLA-4 receptor family. It is essential for cell-cell signaling, immune responses, and regulation of cell proliferation. ICOS is associated with disorders such as Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.