Thermo Fisher Scientific H2A.ZK4ac Recombinant Rabbit Monoclonal Antibody (RM221)
Histone H2A.Z의 Lys4 아세틸화(K4ac)에 특이적인 recombinant rabbit monoclonal antibody. Western blot, ICC/IF, ELISA, Luminex에 사용 가능. 높은 특이성과 재현성을 제공하며, lot 간 일관성 유지. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.05–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM221 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4), with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661888 |
Product Specific Information
This antibody reacts specifically with Histone H2A.Z acetylated at Lysine 4 (K4ac).
It shows no cross-reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems.
These systems clone specific antibody DNA sequences from immunoreactive rabbits, then screen individual clones to select the best candidates for production.
Advantages include:
- Higher specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure in eukaryotic chromatin.
Two molecules each of H2A, H2B, H3, and H4 form an octamer, around which ~146 bp of DNA is wrapped to form nucleosomes.
Linker histone H1 binds linker DNA between nucleosomes, aiding chromatin compaction.
This gene encodes a histone H2A family member and produces two transcripts via distinct termination motifs.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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