Thermo Fisher Scientific H2A.ZK7ac Recombinant Rabbit Monoclonal Antibody (RM222)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-5 µg/mL

-

ELISA (ELISA)

0.2-1 µg/mL

-

ChIP assay (ChIP)

5 µg/mL

-

Luminex (LUM)

0.05-0.5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM222

Immunogen

Acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys7). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H2A.ZK7ac,uniProtId:P0C0S5-1,ncbiNodeId:9606,antigenRange:7,antigenLength:128,antigenImageFileName:MA5-24665_H2A.ZK7ac_P0C0S5-1_Rabbit.svg,antigenImageFileNamePDP:MA5-24665_H2A.ZK7ac_P0C0S5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4, with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2661886

Product Specific Information

This antibody reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.