Thermo Fisher Scientific H2A.ZK7ac Recombinant Rabbit Monoclonal Antibody (RM222)
H2A.ZK7ac에 특이적인 recombinant rabbit monoclonal antibody로 높은 특이성과 재현성을 제공합니다. Western blot, ICC, ELISA, ChIP 등 다양한 응용에 적합합니다. Lot 간 일관성이 우수하며, 동물 유래 성분이 없는 포뮬레이션으로 연구용에 최적화되어 있습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 5 µg/mL |
| Luminex (LUM) | 0.05–0.5 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM222 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys7) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661886 |
Product Specific Information
This antibody reacts specifically to Histone H2A.Z acetylated at Lysine 7 (K7ac). It shows no cross-reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction.
This gene encodes a member of the histone H2A family, generating two transcripts through conserved stem-loop termination and polyA addition motifs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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