Thermo Fisher Scientific Histone H2A.X Recombinant Rabbit Monoclonal Antibody (RM214)
Histone H2A.X 단백질을 인식하는 재조합 토끼 단클론 항체로, 인간 시료에 반응합니다. Western blot, ICC/IF, ELISA, Luminex 등 다양한 응용 가능. 높은 특이성과 민감도, 로트 간 일관성 및 동물 유래 성분 없는 제형 제공.
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Thermo Fisher Scientific Histone H2A.X Recombinant Rabbit Monoclonal Antibody (RM214)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.2–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM214 |
| Immunogen | Peptide corresponding to the C-terminus of human Histone H2A.X |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4) with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661890 |
Product Specific Information
This antibody reacts to Histone H2AX protein, independent of post-translational modifications, and shows no cross-reactivity with other histone proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of four core histones forming the nucleosome core particle.
In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the C-terminus of H2AX, resulting in γ-H2AX. This phosphorylation can be detected by Western blotting or immunofluorescence, indicating DSB frequency.
Phosphorylation is mediated by phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR.
H2A.X consists of 143 amino acids and is synthesized in both G1 and S phases. Its mRNA occurs in two forms: a 1600-base polyA-containing form and a 575-base form lacking polyA.
Histone H2A.X maps to chromosome 11q23.2–q23.3 and contributes to DNA structure formation. The variant H2A.X regulates MDC1 interaction at DNA damage sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: MA5-24663_Histone_H2A.X_P16104-1_Rabbit.svg, MA5-24663_Histone_H2A.X_P16104-1_Rabbit_PDP.jpeg)
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